Fig. 1. E-cadherin is required for germ cell directionally persistent migration track.

a Graph: PGC migration speed and directional persistence in e-cadherin^weg/weg embryos relative to control embryos. n = number of cells from 4 independent experiments. Right: representative tracks of PGCs migrating within e-cadherin^+/+ and ^+/weg embryos or e-cadherin^weg/weg embryos. b Graph: PGC migration speed and directional persistence in e-cadherin-morpholino-treated embryos relative to control embryos. n = number of cells from 4 independent experiments. Right: representative tracks of PGCs migrating within embryos injected with either a control or e-cadherin morpholino. c Graph: migration speed and directional persistence of PGCs expressing a dominant-negative form of E-cadherin as compared with control cells. n = number of cells from 3 independent experiments. Right: representative tracks of cells expressing a control protein or a dominant-negative form of E-cadherin. The results presented in a–c were derived from time-lapse movies captured between 6 and 8 hours post fertilization (hpf) at a time interval of 2 min between frames. The tracks are presented as 2-dimensional Z-projection of the 3-dimensional data acquired. Cells were tracked for 70 min and analyzed using Imaris software. Additional representative tracks are provided in Supplementary Fig. 1a. Normalized mean ± s.e.m.; P value: two-tailed Mann–Whitney U-test; ns = not significant. d Graph: number of cells that reside out of the developing gonad region at 22 hpf. Mean ± s.e.m.; P value: two-tailed Mann–Whitney U-test; N = number of embryos from 3 independent experiments. Right: Dashed yellow line indicates the developing gonad region, which spans the first half of the yolk extension; yellow arrows point at ectopic cells; scale bars, 150 μm. Source data are provided as a Source Data file.