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. 2020 Oct 26;10:18251. doi: 10.1038/s41598-020-75184-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Demonstration of the status of ICG retention in the bladder and urethra. ICG was injected into the bladder. The retained ICG solution was subsequently figured by utilizing in vivo imaging device IVIS. Subsequent amount of retained ICG can be visualized in the bladder and urethra (anterior and posterior). Immediately after the injection, the entire bladder (white arrowhead) was visualized. (A) Later, the urethra (black arrow) was visualized in addition to the bladder. (B) Urine including ICG overflowed from external urethral meatus (C; white arrow).