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. 2020 Oct 26;10:18251. doi: 10.1038/s41598-020-75184-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The urinary tract status including bladder and urethra in vivo was detected by the current procedure of anterograde CA-mediated urethrography. This examination was performed in oblique position with normal laboratory mouse, similar to urethrography for human. Pre-examination image (A) with butterfly needle (yellow arrow) and os penis (yellow arrowhead) before CA injection is shown. Visualization was performed by the CA injection to the bladder through butterfly needle. (B) Bladder (black arrowhead), anterior urethra (white arrow), posterior urethra (black arrow) and urethral diverticulum (white arrowhead) were visualized. The caliber of the posterior urethra was wider than that of the anterior urethra (black and white arrow) and the border between them was distinguished at the urethral diverticulum (white arrowhead). The position of the os penis (yellow arrowhead) was the landmark in distinguishing between the part of penile shaft and grans.