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. 2020 Oct 26;10:18251. doi: 10.1038/s41598-020-75184-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of anterior and posterior urethral status in vivo by the current procedure of anterograde CA injection to the bladder by IVIS imaging. The formation of fistula (black arrowhead) after 2 days of operation are shown (A, B). Histological examination of epithelial status of the operated site (C; green circle) in axial section (C; red line section). Hematoxylin Eosin (D; black arrow) and anti-E-Cadherin (E; white arrow) stain revealed the epithelialization of the elapsed fistula region (D,E). E-Cadherin was also expressed in the native urethra (white arrowhead).