Fig. 2.
Representative dose-response curves used to determine IC50-values for small-molecule AspH inhibitors displaying >80% potency in the initial AspH inhibition screen. Two dose-response curves each composed of technical duplicates were independently determined using SPE-MS AspH inhibition assays. Assay conditions: 50 nM His6-AspH315–758, 1 μM hFX-CP101–119 (Supporting Fig. S1b), 100 μM l-ascorbic acid (LAA), 3 μM 2OG, and 2 μM Fe(II) in 50 mM HEPES buffer (pH 7.5, 20 °C). Data are shown as the mean average of two technical duplicates (n = 2; mean ± SD).