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. Author manuscript; available in PMC: 2020 Oct 27.
Published in final edited form as: Anal Chem. 2020 May 22;92(12):8561–8568. doi: 10.1021/acs.analchem.0c01459

Figure 2. Optimization of one-pot RPA/CRISPR-Cas12a detection in the DAMR system.

Figure 2.

A: Fluorescent monitoring of the one-pot RPA/CRISPR-Cas12a reactions at different incubation times in the DAMR and one-phase system, respectively. B: Series sucrose concentration (0%, 5%, 10%, 20%, 30%, 40%, and 50%) were added to 20 μL RPA bottom phase and tested in the DAMR system. C: Effect of the volume ratios (1: 1:5, 1:2, 1:1, 2:1, 5:1) of the RPA bottom phase and CRISPR-Cas12a top phase on DNA detection in the DAMR system. Error bars denote s.d. (n=3).