Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 21;19:1533033820967475. doi: 10.1177/1533033820967475

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 4. — ZEB2 was directly repressed by miR-377. (A) The predicted wildtype (WT) binding site of miR-377 in ZEB2. Mutant (MUT) with disrupted binding site of miR-377 in ZEB2 was also shown. (B) The effect of miR-377 mimics on the luciferase activity of reporter gene with wild-type or mutant ZEB2 was detected by luciferase reporter assay. ** represent miR-377 mimics vs. Control, P < 0.01. (C) Effect of miR-377 on protein expression of ZEB2 in HCT116 and HT29 cells invasion was detected by western blot. ** represent miR-377 mimics vs. Control or miR-377 inhibitor vs. NC inh, P < 0.01. (D) Expression level of ZEB2 in colon cancer tissues and adjacent non-cancer tissues was detected by qRT-PCR. ** represent Normal vs. Tumor, P < 0.01. (E) Negative correlation between miR-377 and ZEB2 in colon cancer patients.