Skip to main content
. 2020 Oct 21;19:1533033820967475. doi: 10.1177/1533033820967475

Figure 5.

Figure 5.

Over-expression of miR-377 suppressed colon cancer stem cell phenotype and EMT via repression of ZEB2. (A) Effect of miR-377 and ZEB2 on the protein expression of ZEB2, ALDH1, CD133, E-cadherin, N-cadherin and vimentin in HCT116 cells was detected by western blot. ** represents miR-377 + Vector vs. Control + Vector, P < 0.01. ## represents miR-377 + Vector vs. miR-377 + ZEB2, P < 0.01. (B) Effect of miR-377 and ZEB2 on the percentage of ALDH+ cells detected by flow cytometry sorting. ** represents miR-377 + Vector vs. Control + Vector, P < 0.01. ## represents miR-377 + Vector vs. miR-377 + ZEB2, P < 0.01. (C) Effect of miR-377 and ZEB2 on the self-renewal of spherogenic HCT116 cells was detected by sphere formation assay. ** represents miR-377 + Vector vs. Control + Vector, P < 0.01. ## represents miR-377 + Vector vs. miR-377 + ZEB2, P < 0.01. (D) Effect of miR-377 mimics and ZEB2 overexpression on HCT116 cell migration was detected by wound healing assay. ** represents miR-377 + Vector vs. Control + Vector, P < 0.01. ## represents miR-377 + Vector vs. miR-377 + ZEB2, P < 0.01. (E) Effect of miR-377 mimics and ZEB2 overexpression on HCT116 cell invasion was detected by transwell assay. ** represents miR-377 + Vector vs. Control + Vector, P < 0.01. ## represents miR-377 + Vector vs. miR-377 + ZEB2, P < 0.01.