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Primer Used for qPCR
| Gene | Primer Sequence(5ʹ 3ʹ) |
|---|---|
| HO-1 | Forward: CAGACAGAGTTTCTTCGCCAGAGG |
| Reverse: TGTGAGGACCCATCGCAGGAG | |
| Nrf2 | Forward: GCCTTCCTCTGCTGCCATTAGTC |
| GAPDH | Reverse: TGCCTTCAGTGTGCTTCTGGTTG Forward: TGCACCACCAACTGCTTAG Reverse: GATGCAGGGATGATGTTC |
Note: All primers were designed by TaKaRa, Japan.
Abbreviations: HO-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2-related factor 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
