FIG 1.

Accuracy of single cell analysis. (A) Structure of rDNA in mouse. One unit of rDNA is ∼43 kb, composed of the 45S pre-rRNA gene and intergenic spacer. 45S pre-rRNA is subsequently processed into three mature rRNAs (18S, 5.8S, and 28S). (B) rDNA copy numbers of one, two, and four cells were measured by qPCR. The rDNA copy number in RPE1 cells determined by digital PCR was used as the standard (see Materials and Methods). In all, 12 samples for RPE1 and 36 samples (represented by dots) for bone marrow cells were tested. The horizontal bars indicate the averages, and vertical bars indicate SDs.