TABLE 3.

Primer list

Primer name	Sequence (5′–3′)	Note(s) on usea
28S_Fw	TGGGTTTTAAGCAGGAGGTG	Fig. 2 and 4, ddPCR
28S_Rv	GTGAATTCTGCTTCACAATG	Fig. 2 and 4
28S_ddPCR_Rv	GACGGTCTAAACCCAGCTCA	ddPCR
probe_28S_Fw	GTTGCCATGGTAATCCTGCT	Fig. 3, 5, and 6
probe_28S_Rv	ACCCAGAAGCAGGTCGTCTA	Fig. 3, 5, and 6
probe_Swi5_Fw	AGGAGTTGATTCTCTCTACC	Fig. 3 and 6
probe_Swi5_Rv	GCATCAAGACAATTGTGGTT	Fig. 3 and 6
45S_Fw	CTCTTAGATCGATGTGGTGCTC	Fig. 4
45S_Rv	GCCCGCTGGCAGAACGAGAAG	Fig. 4
Actb_Fw	GACGGCCAGGTCATCACTATTG	Fig. 4
Actb_Rv	AGTTTCATGGATGCCACAGG	Fig. 4
GAPDH_Fw	ACTCACGGCAAATTCAACGG	Fig. 4
GAPDH_Rv	ATGTTAGTGGGGTCTCGCTC	Fig. 4
B2M_Fw	TACGTAACACAGTTCCACCC	Fig. 4
B2M_Rv	TGCTGAAGGACATATCTGAC	Fig. 4
18S_Seq_Fw	TAAGAGAGGTGTCGGAGAGC	Fig. 7 and 9
18S_Seq_Rv	CTTCTCTCACCTCACTCCAG	Fig. 7 and 9
5.8S_Seq_Fw	GTCGTTCCCGTGTTTTTCCG	Fig. 7 and 9
5.8S_Seq_Rv	GACCGAGAAAGACTGGTGAG	Fig. 7 and 9
28S_Seq_Fw	GGTTGCGTGTGAGTAAGATCCTC	Fig. 7 and 9
28S_Seq_Rv	TACTGGTCGACCTCCGAAGTTG	Fig. 7 and 9
ATP5b_Seq_Fw	GAATAATGGCGGTTCTGTGCAC	Fig. 7 and 9
ATP5b_Seq_Rv	ATGATTCTGCCCAAGGTCTCAG	Fig. 7 and 9
28S_target_probe	6FAM-GCCGACATCGAAGGATCAAAAAGCGAC-BHQ1	ddPCR

^aFigure references are given for the relevant experiments.