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. 2020 Oct 21;18(10):522. doi: 10.3390/md18100522

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Resistance acquisition during 21 serial passaging (21 days) in the presence of sub-MIC (0.5 × the MIC determined from the previous passage) levels of ciprofloxacin, chromomycin A₉ (1), Ap (2), A₂ (3), and A₃ (4) for S. aureus ATCC43300. The y axis is the highest concentration the cells grew to during passaging. For ciprofloxacin, 128 × MIC was the highest concentration tested. The figures are representative of three independent experiments.