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. 2020 Oct 19;21(20):7719. doi: 10.3390/ijms21207719

Figure 1.

Figure 1

Effect of primed vs. naive CD4+ T cells specific for a viral determinant on the development of TMEV-induced demyelinating disease. (A) Control SJL and VP2-TCR-Tg mice were infected with TMEV (2 × 106 pfu/mouse), and the development of clinical symptoms was compared between the groups over 60 days. The two-tailed p values between the groups were significant based on a paired t test of the mean clinical cores between days 9 and 60 postinfection: p < 0.0001 (t = 9.739 with 8 degrees of freedom) between the VP2-TCR-Tg group and the control SJL group. (B) Proportions of IFN-γ producing CD4+ T cells in the SJL and VP2-TCR-Tg mice. After 8 days of infection, CNS infiltrating cells were restimulated with PBS, anti-CD3/CD28, 2 µM VP272-86, or 3D20-38 peptides for 6 hr. The proportions of CD4+ T cells producing IFN-γ and IL-17 were determined using flow cytometry. (C) The numbers of CD4+ T cells producing IFN-γ and IL-17 in the CNS of TMEV-infected SJL and TCR-Tg mice at 8 dpi. ** p < 0.001.