Figure 3.
The gating strategy of CD4+ T cells, CD8+ T cells, and B cells in spleen and mesenteric lymph nodes (MLNs). Spleen and MLN cells from mice (n = 6) were collected at 35 dpi upon challenge infection with T. gondii ME49 and stained with phenotype-specific antibodies (CD3, CD4, CD8, B220, and GL7). The gating strategies depicted above were applied to identify CD4+ and CD8+ T cells (A,B), germinal center B cells, and B cells from both spleen and MLNs (C–F).