Figure 5.

Immunization with the adjuvanted T. gondii VLPs enhances antigen-specific B-cell responses. Immunized mice (n = 6 per group) were infected with T. gondii ME49, 4 weeks after the last immunization. Spleen and MLN tissues were harvested at 35 dpi and then cell phenotypes were determined by flow cytometry. The percentage of GC B (A,C) cells or B cells (B,D) was quantified by subtracting unstimulated cells from stimulated cells. Results are presented as mean ± SD and statistical significances were denoted using asterisks (** p < 0.01 and *** p < 0.001).