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. 2020 Oct 21;8(10):1627. doi: 10.3390/microorganisms8101627

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Confocal microscopy images of Candida albicans cells treated with fluorescein isothiocyanate (FITC)-labeled K13L for 5 min (panel A), 75 min (panel B), 285 min (panel C), and 315 min, 30 min after addition of propidium iodide (PI) (panel D, FITC, panel E, PI, panel F, merge). Some non-viable yeast cells are already present in the inoculum (panel A, arrows); over time, intracellular localization of FITC-labeled K13L is observed (some cells indicated by arrowheads, panels B and C). Peptide internalization led to cell death, as demonstrated by the merge of green (FITC) and red (PI) signals in panel F).