Skip to main content
. 2020 Oct 12;21(20):7523. doi: 10.3390/ijms21207523

Figure 7.

Figure 7

Effect of chrysoeriol on the inhibition of MAPK and CYP19 expression. (A,B) Serum-starved MCF-7 cells were pretreated with 5 μM U0126, 10 μM SP600125, or 10 μM SB203580 for 30 min, followed by treatment with 10 ng/mL TNFα. After 24 h, cells were harvested and CYP19 mRNA expression was examined by RT-PCR (A) and qR-PCR (B). GAPDH was used as an internal control. (C) Serum-starved MCF-7 cells were pretreated with chrysoeriol (0, 10, and 20 μM) for 30 min, followed by treatment with 10 ng/mL TNFα. After 10 min (phospho-ERK1/2) or 20 min (phospho-JNK1/2 and phospho-p38), cells were harvested, and immunoblotting was performed using antibodies against phospho-ERK1/2 (Thr202/Tyr204), phospho-JNK1/2 (Thr183/Tyr185), and phospho-p38 kinase (Thr180/Tyr182). Band intensities were measured using the ImageJ software, and phospho-proteins were normalized to corresponding total proteins. Bars represent the mean ± SD (n = 3). NS, not significant; * p < 0.05, *** p < 0.001 by Dunnett’s multiple comparisons test. qR-PCR, quantitative real-time PCR.