Table 3.
Primer sequences used for SYBR green real-time PCR.
| Gene | Primer | Sequence 5′→ 3′ |
|---|---|---|
| β-ACT | forward | TGGTTACAGGAAGTCCCTTGCC |
| β-ACT | reverse | ATGCTATCACCTCCCCTGTGTG |
| CYP27B1 | forward | GGAACCCTGAACAACGTAGTC |
| CYP27B1 | reverse | AGTCCGAACTTGTAAAATTCCCC |
| TGM2 | forward | CCTTACGGAGTCCAACCTCA |
| TGM2 | reverse | CCGTCTTCTGCTCCTCAGTC |
| TNF-α | forward | GTGAGGAGGACGAACATC |
| TNF-α | reverse | GAGCCAGAAGAGGTTGAG |
| IFN-γ | forward | GCAGCCAACCTAAGCAAGAT |
| IFN-γ | reverse | TCACCTGACACATTCAAGTTCTG |
| LC3 | forward | CGGTGATAATAGAACGATACAAG |
| LC3 | reverse | CTGAGATTGGTGTGGAGAC |
| ATG5 | forward | TGCCTGAACAGAATCATCCTT |
| ATG5 | reverse | CCAGCCCAGTTGCCTTAT |
| BECN1 | forward | ACAGTGAACAGTTACAGATGGA |
| BECN1 | reverse | CTCAGCCTGGACCTTCTC |
Abbreviations: β-ACT, β-actin; CYP27B1, 1α-hydroxylase; TGM2, tissue transglutaminase; TNF-α, tumor necrosis factor-α; IFN-γ, interferon-γ; LC3, microtubule-associated protein 1A/1B-light chain 3; ATG5, autophagy-related 5; BECN1, Beclin 1.