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. 2020 Oct 19;8(10):1606. doi: 10.3390/microorganisms8101606

Figure 1.

Figure 1

Preparation of Material (A) and Material (B1–B6). (a) To produce Material (A), Material (A1) was mixed with an equal weight of Material (A1-1) and Material (A1-2). Material (A1-1) was a dried and pulverized mixture of 10% (w/w) field thistle (leaf part, stem part, and flower part), 60% (w/w) mugwort (leaf part and stem part), and 30% (w/w) Farfugium japonicum (leaf part and stem part). Material (A1-2) was a dried and pulverized mixture of 20% (w/w) Rosa multiflora (leaf part, flower part), 10% (w/w) Geum japonicum (leaf part and stem part), and 70% (w/w) raspberry (leaf part, stem part, and flower part). Material (A2) was a dried mixture of 25% (w/w) Acer (leaf part and stem part), 25% (w/w) Betula platyphylla var. japonica (leaf part, stem part, and bark part), and 50% (w/w) Cryptomeria japonica (leaf part, stem part, and bark part). Then, Material (A1) and Material (A2) was mixed at a ratio of 1:3 to obtain Material (A). (b) To produce Material (B), various combinations of limestone, fossil coral, shell, and activated carbon were used to obtain Material (B1), Material (B2), Material (B3), Material (B4), Material (B5), and Material (B6). Material (B1) comprised 70% (w/w) limestone, 15% (w/w) fossil coral, and 15% (w/w) shell. Material (B2) comprised 40% (w/w) limestone, 15% (w/w) fossil coral, 40% (w/w) shell, and 5% (w/w) activated carbon. Material (B3) comprised 80% (w/w) limestone, 15% (w/w) fossil coral, and 5% (w/w) shell. Material (B4) comprised 90% (w/w) limestone, 5% fossil coral, and 5% shell. Material (B5) comprised 80% (w/w) limestone, 10% (w/w) fossil coral, and 10% (w/w) shell. Material (B6) comprised 60% (w/w) limestone, 30% (w/w) fossil coral, and 10% (w/w) shell.