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Experimental set-up. (a) Seven-day-old chicken embryo. After cutting a small window into the upper part of the eggshell, the window was closed with Parafilm®, and the eggs were further incubated. The in ovo CAM assay allows direct access to the extraembryonic chorioallantoic membrane via the open window. (b) Through the window, the ingrowth of soft tissue and vessels into the porcine-derived CM can be monitored.
