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. 2020 Oct 13;21(20):7555. doi: 10.3390/ijms21207555

Figure 1.

Figure 1

Expression of chemerin isoforms in LX-2 cells. (a–c) LX-2 cells were transfected with an insertless plasmid (C) or plasmids to express huChem-155, 156 or 157. Cellular chemerin protein was analyzed by immunoblot at 24, 48 and 72 h post-transfection. Coomassie stained membrane served as control; (d–f) Soluble chemerin protein was analyzed by ELISA at 24, 48 and 72 h post-transfection; (g–i) Chemerin mRNA was analyzed by real-time PCR at 24, 48 and 72 h post-transfection. N = 3. * p < 0.05, ** p < 0.01, *** p < 0.001.