Figure 1.

Methionine sulfoxide reductase (Msr)B1 is needed for normal lipopolysaccharide (LPS)-induced maturation of dendritic cells (DCs). Bone marrow-derived DCs (BMDCs) from wild-type (WT) and MsrB1^−/− mice were stimulated with LPS for the indicated durations (n = 3 per group) and their expression of CD80 (A), CD86 (B), CD40 (C), major histocompatibility complex (MHC) II (D), and PD-L1 (E) were analyzed by flow cytometry with gating on the CD11c⁺CD11b⁺ BMDCs in the live cells. In all figures, MFI stands for mean fluorescence intensity. Fluorescence minus one (FMO) controls (gray) are shown in each histogram. Mean ± SEM are shown. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant, as determined by unpaired t-test. The data shown are from one of four independent experiments, all of which had similar results.