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. 2020 Oct 20;9(10):1021. doi: 10.3390/antiox9101021

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MsrB1 promotes the production of IL-12 by DCs in vitro. (A) WT and MsrB1^−/− BMDCs were stimulated with LPS for the indicated times (n = 3 per group) and their transcript levels of IL-12a, IL-12b, TNF, IL-1b, IL-6, and IL-10 were analyzed by qRT-PCR. (B) The intracellular IL-12p40/70 levels in WT and MsrB1^−/− BMDCs at the indicated time points after LPS stimulation (n = 3 per group) were measured by flow cytometry with gating on the CD11c⁺CD11b⁺ cells in the live cells. (C) The extracellular levels of IL-12p70 in the BMDC cultures were measured by ELISA (n = 3 per group). Mean ± SEM are shown. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant, as determined by unpaired t-test. The data are representative of two experiments, which had similar results.