Figure 5.

MsrB1 promotes the LPS-induced IL-12 production of classical splenic DCs in vivo. (A–D). Splenocytes isolated from WT and MsrB1^−/− mice 3 h after LPS injection were cultured in the presence of Brefeldin A for 4 h. The CD8α⁺ (A,C) and CD11b⁺ (B,D) DCs of the WT and MsrB1^−/− mice (n = 6 per group) were analyzed by flow cytometry for their intracellular IL-12 p40/70 levels (A,C) and their expression of CD80, CD86, and MHC II (B,D). The classical DCs were analyzed by gating on the CD8α⁺ or CD11b⁺ cells in the live CD11c⁺ MHC II⁺ cells. Mean ± SEM are shown. * p < 0.05, ** p < 0.01; ns, not significant, as determined by Mann-Whitney U test. The data are representative of two experiments, which had similar results.