Figure 6.

MsrB1 promotes the DC-induced differentiation of T-helper (Th)1 cells. (A) WT or MsrB1^−/− BMDCs were loaded with 50 μg/mL OVA and cocultured with OT-II cells for 3 days, after which the intracellular IFN-γ levels of the SSC^lowTCRβ⁺ OT-II cells were measured by flow cytometry (n = 3 per group). (B) Schematic depiction of the in vivo model that was used to assess the effect of MsrB1 deficiency on the ability of DCs to promote Th1 differentiation. Thus, 1 × 10⁶ CFSE-labeled CD4⁺ OT-II cells were adoptively transferred into WT and MsrB1^−/− mice, which were then injected intraperitoneally with OVA and LPS. Three days later, the OT-II cells in the spleens of the WT and MsrB1^−/− recipients (n = 5 per group) were assessed by flow cytometry for CFSE dilution (C), CD44⁺ expression (D), and intracellular IFN-γ expression (E). The transferred OT-II cell population was analyzed by gating on the Vα2⁺CFSE⁺ cells in the live CD3⁺CD4⁺ cells. Mean ± SEM are shown. * p < 0.05, ** p < 0.01; ns, not significant, as determined by Mann-Whitney U test. The data are representative of two experiments, which had similar results.