Figure 7.

MsrB1 may promote follicular helper T-cell (Tfh) differentiation in vivo. (A) Schematic depiction of the sheep red blood cell (SRBC) immunization protocol. WT and MsrB1^−/− mice (n = 6 per group) were injected intraperitoneally with SRBC and the splenocytes were isolated 7 days later. (B) The Tfh (PD-1^highCXCR5^high CD4⁺) cells in the spleen of the WT and MsrB1^−/− mice were measured by flow cytometry by gating on the CD3⁺CD4⁺ cells in the live cells. Mean ± SEM are shown. * p < 0.05; ns, not significant, as determined by the Mann-Whitney U test. The data are representative of two experiments, which had similar results.