Figure 3.

Mutations in Kir2.1 diminish the actin reorganization effect. (A) The dynamics of actin filaments in Kir2.1-overexpressed cells imaged by SIM. Hela cells were transfected with lifeact-EGFP and mCherry-fused Kir2.1. Scale bar: 5 μm. (B) The dynamics of actin filaments in the Kir2.1-K188Q-overexpressed cells imaged by SIM. Hela cells were transfected with lifeact-EGFP and mCherry-fused Kir2.1-K188Q. Scale bar: 5 μm. (C) Quantification of the dynamic actin filament ratio. Representative images were shown in (A) and (B). n = 3–4 cells. Values are mean ± SEM. ** p < 0.01. (D) The dynamics of actin filaments in Kir2.1-overexpressed cells before PIP₂ depletion. Hela cells were transfected with lifeact-EGFP and mCherry-fused Kir2.1. Scale bar: 5 μm. (E) The dynamics of actin filaments in Kir2.1-overexpressed cell after PIP₂ depletion. PLC agonist (10 µM m-3M3FBS) and PI3K inhibitor (100 nM wortmanin) were used as PIP₂ depletion agents. Hela cells were transfected with lifeact-EGFP and mCherry-fused Kir2.1. Scale bar: 5 μm. (F) Quantification of the ratio of dynamic actin filaments in Kir2.1-overexpressed cells before and after PIP₂ depletion. Representative images were shown in (D) and (E). n = 3 cells. Values are mean ± SEM. ** p < 0.01.