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. 2020 Oct 19;12(10):3045. doi: 10.3390/cancers12103045

Figure 2.

Figure 2

Ex vivo immune responses against mutant epitopes in three different healthy donors, with the wild-type (wt) epitope and unstimulated cells as negative controls. Healthy donor peripheral blood mononuclear cells were thawed and rested over night before they were plated directly ex vivo into the ELISPOT plate and stimulated with the appropriate peptides for 48 h before development of the plate. The experiments included both wt peptide-stimulated cells and unstimulated cells as negative controls. Response to G12D (A), G13V (B), and G12A (C) epitopes are shown. * indicates a DFR-defined response, whereas ** indicates a DFR(2x) defined response [28].