Figure 5.

Exopolysaccharide production by the wild type RtTA1 and ΔpssJ, ΔpssJ(pssJ), and ΔpssJ(pssJ-his6) strains. (A) The total amount of EPS precipitated with 4 volumes of ethanol from the supernatants of cultures grown for 5 days in the 79CA medium containing 0.5% glycerol. Bars labeled with different letters represent values that are significantly different at p < 0.05. The amount of EPS is expressed as glucose equivalents. (B) Gel permeation chromatography of exopolysaccharides; the retention times of dextran blue (2 MDa) and dextran T10 (10 kDa) (molecular mass markers) are shown as blue dotted lines. Monosaccharide molar ratios in exopolysaccharides precipitated from supernatants are shown in the boxes on the right; the ratio specific for the mutant, indicating absence of galactose in EPS, is shown in dark blue.