Figure 9.
Swimming motility and biofilm formation of the wild type RtTA1 and its derivatives. Swimming motility was measured as the diameter of the zone around the puncture with bacterial suspension on days 4, 8, and 12 after inoculation into media solidified with 0.3% agar (A). Biofilm formation was examined by staining with crystal violet and expressed as the ratio of the amount of crystal violet solubilized by ethanol to the bacterial growth (OD560/OD600) (B). Bars of standard deviation labeled with different letters represent values significantly different at p < 0.05.