Table 1.
Strains and plasmids used in this work.
| Strain/Plasmid | Relevant Description | Reference |
|---|---|---|
| Escherichia coli | ||
| S17-1 | 294 derivative RP4-2-Tc::Mu-Km::Tn7 chromosomally integrated | [52] |
| DH5α | supE44 ΔlacU169 (Δ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [53] |
| DHM1 | Reporter strain for BTH system; F- glnV44(AS) recA1 endA gyrA96 thi-1 hsdR17 spoT1 rfbD1 cya-854 | [54] |
| BL21(DE3) | F– ompT hsdSB (rB- mB-) gal dcm (DE3) | Novagen |
| Rhizobium leguminosarum bv. trifolii | ||
| RtTA1 | wild type strain, StrR, RifR | [55] |
| TA1ΔpssJ(GmR) | RtTA1 ΔpssJ::GmR | This work |
| TA1ΔpssJ[pCM157] | RtTA1 ΔpssJ carrying pCM157 cre expressing vector | This work |
| TA1ΔpssJ | RtTA1 ΔpssJ | This work |
| TA1ΔpssJ[pBKpssJ-C] | ΔpssJ carrying pBKpssJ-C (complemented mutant) | This work |
| TA1ΔpssJ[pBKpssJ-C-His6] | ΔpssJ carrying pBKpssJ-C-His6 (complemented mutant expressing His6-tagged PssJ) | This work |
| Plasmids | ||
| pET30c | ori pBR322, KanR, lacI, 6xHis-tag, S-tag | Novagen |
| pET30c-pssJ | pET30c derivative with 827-bp fragment comprising pssJ gene cloned in BamHI – XhoI | This work |
| pCM351 | ori ColE1, oriT, ApR, GmR, TcR, allelic exchange vector | [39] |
| pCM157 | ori IncP, oriT, TcR, cre expression vector | [39] |
| pBBR1MCS-2 | pBBR1 rep, mob, lacZα multi cloning site, KmR, broad-host-range cloning vector | [56] |
| pCGpssJ-U | pCM351 with 700 bp NdeI fragment comprising last 688 bp of pssK and pssK–pssJ intergenic region | This wok |
| pCGpssJ-UD | pCGpssJ-U with 570 bp ApaI–SacI fragment comprising pssJ–pssI intergenic region and 487 bp of pssI | This wok |
| pBKpssJ-C | pBBR1MCS-2 with 990 bp ApaI–XbaI fragment comprising last 88 bp of pssK, pssK–pssJ intergenic region, pssJ, and 68 bp downstream of pssJ | This work |
| pBKpssJ-C-His6 | pBBR1MCS-2 with 940 bp ApaI–XbaI fragment comprising last 88 bp of pssK, pssK–pssJ intergenic region, and pssJ without stop codon, equipped with His6-tag coding sequence and TAA stop codon | This work |
| pUT18 | Two-hybrid plasmid for cyaAT18 fusion construction, Apr | [54] |
| pUT18C | Two-hybrid plasmid for cyaAT18 fusion construction, Apr | [54] |
| pKNT25 | Two-hybrid plasmid for cyaAT25 fusion construction, Kmr | [54] |
| pKT25 | Two-hybrid plasmid for cyaAT25 fusion construction, Kmr | [54] |
| pUT18C-zip | Two-hybrid control plasmid, Apr | [54] |
| pKT25-zip | Two-hybrid control plasmid, Kmr | [54] |
| pUT18-pssA/pssC/pssD/pssE/pssF/pssG/pssH/pssI | pUT18 with DNA fragment of 792 bp, 981 bp, 459 bp, 477 bp, 885 bp, 975 bp, 969 bp, 945 bp comprising pssACDEFGHI genes cloned into XbaI-KpnI sites | This work |
| pUT18-pssS | pUT18 with DNA fragment of 1152 bp comprising pssS gene, cloned into XbaI-BamHI sites | This work |
| pUT18-pssJ | pUT18 with DNA fragment of 822 bp comprising pssJ gene, cloned into PstI-BamHI sites | This work |
| pUT18C-pssA/pssC/pssD/pssE/pssF/pssG/pssH/pssI | pUT18C with DNA fragment of 792 bp, 981 bp, 459 bp, 477 bp, 885 bp, 975 bp, 969 bp, 945 bp comprising pssACDEFGHI genes cloned into XbaI-KpnI sites | This work |
| pUT18C-pssS | pUT18C with DNA fragment of 1152 bp comprising pssS gene, cloned into XbaI-BamHI sites | This work |
| pUT18C-pssJ | pUT18C with DNA fragment of 822 bp comprising pssJ gene, cloned into PstI-BamHI sites | This work |
| pKT25-pssA/pssC/pssD/pssE/pssF/pssG/pssH/pssI | pKT25 with DNA fragment of 792 bp, 981 bp, 459 bp, 477 bp, 885 bp, 975 bp, 969 bp, 945 bp comprising pssACDEFGHI genes cloned into XbaI-KpnI sites | This work |
| pKT25-pssS | pKT25 with DNA fragment of 1152 bp comprising pssS gene, cloned into XbaI-BamHI sites | This work |
| pKT25-pssJ | pKT25 with DNA fragment of 822 bp comprising pssJ gene, cloned into PstI-BamHI sites | This work |
| pKNT25-pssA/pssC/pssD/pssE/pssF/pssG/pssH/pssI | pKNT25 with DNA fragment of 792 bp, 981 bp, 459 bp, 477 bp, 885 bp, 975 bp, 969 bp, 945 bp comprising pssACDEFGHI genes cloned into XbaI-KpnI sites | This work |
| pKNT25-pssS | pKT25 with DNA fragment of 1152 bp comprising pssS gene, cloned into XbaI-BamHI sites | This work |
| pKNT25-pssJ | pKT25 with DNA fragment of 822 bp comprising pssJ gene, cloned into PstI-BamHI sites | This work |
| pMEG65 | Vector with constitutively expressed gfp and RK2 stabilization fragment, Tcr | [57] |