Figure 2.

The interaction between FaRAV1 and the FaMYB10 promoter. a, Autoactivation was tested on SD/‐Ura in the presence of 125 ng/mL aureobasidin A (AbA). b, Physical interaction was determined on SD medium lacking Leu in the presence of 125 ng/mL AbA. The empty pGADT7 vector was applied as a negative control. c, The probe used for EMSA with the RAV core sequence is in red, and the mutated nucleotides indicated in green. D, EMSA of 3′‐biotin‐labelled dsDNA probes with the purified FaRAV1 protein. The presence (+) or absence (–) of specific probes is marked. The concentration of the cold probe was 16 nm (+), 32 nm (++) or 64 nm (+++) while that of the biotinylated probe was 8 nm. Water was added in place of FaRAV1 protein as a control.