Figure 1.

a) N ⁶‐isopentenyladenosine (red) in comparison to unmodified adenosine (blue, only nucleobases shown). b) RNA‐cleaving deoxyribozymes catalyse the cleavage of the phosphodiester backbone by transesterification. c–e) Depiction of three different classes of DNAzymes identified in this study; AA DNAzymes are inhibited by i⁶A (c), the AB08 DNAzyme cleaves only i⁶A‐modified RNA (d). The cleavage site of the AC17 DNAzyme is influenced by the modification state of the RNA; that is, modified and unmodified RNA are cleaved at different positions (e).