Figure 2.

a) Sequences and predicted secondary structures of three AA DNAzymes, AA07, AA14, and AA17. b) PAGE analysis of the cleavage reaction with unmodified RNA (R1, labelled with blue −) and i⁶A‐modified RNA (R2, labelled with red +) shows selective cleavage of unmodified RNA, and identifies the cleavage site; AA07 and AA14 cleave at G15, and AA17 cleaves at G16. Experiments used 3′‐fluorescein‐labelled RNA. c) Single‐turnover kinetics for AA17. Graphs for others and gel images are shown in Figure S3. d) HR‐ESI‐MS analyses of cleaved products. The longer 15/16‐nt products contain a 2′,3′‐cyclic phosphate, and the shorter 12/13‐nt fragments contain 5′‐OH and 3′‐C₆‐NH₂‐termini.