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. 2020 Jul 4;18(11):2354–2363. doi: 10.1111/pbi.13400

Figure 4.

Figure 4

APIP4 trypsin inhibitor activity is differentially modulated in the AvrPiz‐t and Piz‐t‐HA transgenic plants. (a) APIP4 trypsin inhibitor activity is suppressed in the AvrPiz‐t transgenic plants. (b) Transcript levels of APIP4 were measured by RT‐PCR in two‐week‐old NPB and AvrPiz‐t plants. The Ubiquitin gene UBQ was used as the internal control. (c) APIP4 protein levels were determined in two‐week‐old NPB and AvrPiz‐t plants by immunoblotting using the anti‐APIP4 antibody. The HSP protein was used as the internal control. (d) Enhancement of APIP4 trypsin inhibitor activity by Piz‐t. (e) Transcript levels of APIP4 were measured by RT‐PCR in two‐week‐old NPB and Piz‐t‐HA plants. The Ubiquitin gene UBQ was used as the internal control. (f) APIP4 protein levels were determined in two‐week‐old NPB and Piz‐t‐HA plants by immunoblotting using the anti‐APIP4 antibody. The HSP protein was used as the internal control.