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. 2020 Oct 9;21(20):7448. doi: 10.3390/ijms21207448

Figure 3.

Figure 3

Dose–response curve of ISO1 given at 30 min after FPI, examining immune cell penetration into the brain at 24 h after FPI. We administered ISO1 at 30 min after FPI and performed flow cytometry on leukocytes isolated from the brain to ascertain the optimum dose of ISO1 and to assess what percentage of isolated are CLIP+ B cells. It should be noted that very few cells were isolated from naïve mice, so that was removed from the graph. Cells were stained with anti-CD19 and counter-stained with Anti-MHC class II invariant chain CLIP. The results indicate that a 10 mg/Kg dose of ISO1 administered 30 min after FPI provided the most robust decrease in % of B cells that express CLIP (* p < 0.05).