Figure 4.

Effects of ISO1 on the frequency γδ T cells on the gut after FPI. We administered ISO1 using 10 mg/Kg at 30 min after FPI, and performed flow cytometry on leukocytes isolated from either proximal or distal intestine 24 h following FPI to assess what percentage of isolated leukocytes are γδ T cells. T cells were stained with anti-CD3 antibody and counter-stained with anti-γδ antibody. The percent of T cells that are γδ T cells are significantly increased in the gut after FPI. One way ANOVA * p < 0.05. N = 6 per group, error bars = SD.