Figure 2.

TILLING analysis of the CRT1a locus BnaA09g15400D. (a) Genomic structure consisting of 12 exons (boxes) with the EST region identified in the SSH library indicated in red (see also Figure S4A). (b) Region selected for TILLING containing exons 4–9. (c) Location of mutations identified by TILLING according to the cDNA and amino acid level. (d) CRT1a oilseed rape TILLING mutants were infected with Verticillium longisporum in comparison with the susceptible line Express 617 and a less susceptible NPZ‐resistant reference. Photographs were taken at 35 dpi. Squares are 4 × 4 cm. (e) Rating of disease symptoms. The disease rating was conducted according to Zeise and von Tiedemann (2002) and used to monitor disease severity in infected and non‐infected oilseed rape plants. The rating is distributed from 1 (no symptoms) to 9 (dead plant).