Fig. 1.

Overexpressed cis‐golgins form condensates. (A) HeLa cells were fixed and immunostained for endogenous golgins or (B) HeLa cells were transfected with GM130‐FLAG/Myc, golgin160‐FLAG/Myc, or GMAP210‐FLAG/Myc, respectively, fixed 6‐24 h post‐transfection, and immunostained for FLAG (exogenous golgin). Secondary antibody conjugated to Alexa Fluor 488 was used in (A) and (B). Overexpressed GM130 forms condensates in the nucleus, while golgin160 and GMAP210 form condensates in the cytoplasm. (C) Mean condensate area and (D) mean condensate circularity, where N denotes the number of condensates analyzed and error bars denote the standard error of the mean (SEM). Only condensates with area >1.5 µm² were considered in this analysis, in order to retain an accurate measure for circularity. Scale bars: 5 µm