Representative Sstr4 mRNA expression counterstained with DAPI in the primary nociceptive spinothalamic system in WT mice. Sstr4 mRNA (red, upper and lower panel) co-localized with Rbfox3 mRNA (NeuN, white, lower panel) and are shown in the L5 dorsal root ganglion (DRG) (A). Sstr4 mRNA (red) is shown in the in the laminae V-VII of the spinal cord of L4–L6 ((B)), in the trigeminal ganglion (TG, (C)) co-localized with Rbfox3 mRNA (NeuN, white), and in the ventral posterolateral thalamic nucleus (VPL, Bregma −1.46 mm, (D)) co-localized with Vglut1 (green) mRNA. Note that Sstr4 cannot be detected in VPL area. N = 3. Scale bar 50 µm, inset scale bar: 10 µm.