Representative Sstr4, Vglut1, Gad1 and Chat mRNA expression in various brain areas of WT mice. Sstr4 (red), Vglut1 (green), and Gad1 mRNA (white) expression counterstained with DAPI are shown in the prelimbic cortex (PrL, Bregma 1.54 mm, (A)), in the basomedial amygdala (BMA, Bregma −0.9 mm, (B)), in the core of central amygdala (CeC, Bregma −0.9 mm, (C)), in the basolateral amygdala (BLA, Bregma −1.46 mm, (D)), in the CA1 of hippocampus (CA1, Bregma −1.46 mm, (E)) (N = 5). Sstr4 (red) and Vglut1 (green) expression counterstained with DAPI are shown in the medial nucleus of habenula (MHb, Bregma −1.46 mm, N = 3, (F)). Sstr4 (red) and Chat (green) expression counterstained with DAPI are shown in the MHb (N = 2, (G)). Scale bar: 50 µm, inset scale bar: 10 µm.