Figure 4.

Effect of the administration of AST and injection of ISO on the concentrations of the superoxide anion, cardiolipin (CL) and Mn-SOD2 in rat heart mitochondria under mPTP opening. (a) Superoxide anion production in the presence of EGTA and threshold [Ca²⁺]; (b) alterations in the level of Mn-SOD2 under control and threshold [Ca²⁺] conditions; (c) alterations in the level of CL. * p < 0.05 indicates a significant difference in the protein level relative to the control (group 1). # p < 0.05 compared with RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of the mean values was evaluated using an ANOVA type 2 (Student–Newman–Keuls) test.