Figure 5.

Effect of the administration of AST and injection of ISO on the Ca²⁺ retention capacity and membrane potential in rat heart mitochondria under mPTP opening.(a–d) Alterations in Ca²⁺ and TPP⁺ fluxes in rat heart mitochondria isolated from each group of rats; aliquots of mitochondrial suspension were taken at the points shown on curves a–d for further solubilization of Laemmli buffer and were subjected to SDS–PAGE and immunoblot analysis; (e) quantitative analysis of the Ca²⁺ capacity in rat heart mitochondria isolated from the rats of each group; (f) quantitative analysis of the rate of TPP⁺ influx in rat heart mitochondria isolated from the rats of each group. The data are presented as the means ± SDs of five independent experiments. * p < 0.05 indicates a significant difference in the protein level relative to the control (group 1). # p < 0.05 compared with RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using an ANOVA type 2 (Student–Newman–Keuls) test.