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. 2020 Oct 26;10:18293. doi: 10.1038/s41598-020-75108-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The RNA content of S. aureus MVs consists of Benzonase-sensitive and Benzonase-resistant sub-populations. (A) Bioanalyzer RNA profile of untreated MVs [1], RNA profile of MVs treated with Benzonase [2], and RNA profile of heated MVs treated with detergent, Proteinase K, and Benzonase [3]. X-axis represents the RNA nucleotide length and the y-axis represents fluorescent units. (B) Bioanalyzer gel image of RNA extracted from MV samples treated under different conditions. M indicates the relative migration of RNA standards. The complete gel image is presented in Supplementary Fig. S13A. (C) RNA concentrations from each MV treatment were averaged from triplicate experiments. The data is represented as mean SEM from n = 3. Profiles are representative of two independent experiments. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test (n.s = not significant; ****p < 0.0001).