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. 2020 Oct 26;11:5406. doi: 10.1038/s41467-020-18961-0

Fig. 1. Nod2 plays an unexpected protective role in uveitis.

Fig. 1

Six to ten week old WT and Nod2−/− mice were immunized and assessed weekly thereafter for disease. a Representative fundus images of the posterior pole at day 21 post-immunization, and (b) clinical grading of uveitis based on fundoscopy. Data in b is combined from 6 independent experiments with an n = 12 mice/group for day 7 and day 17, n = 24 (WT) and 19 (Nod2−/−) mice/group for day 10, n = 44 (WT) and 28 (Nod2−/−) mice/group for day 14, and n = 56 (WT) and 54 (Nod2−/−) day 21; *p = 0.0177 (14 day), *p = 0.0093 (day 17), and ***p < 0.0001 (day 21). c Representative epifluorescence images and (d) quantification of blood-retinal barrier integrity 10 days post-immunization. Data is combined from 2 independent experiments where n = 9 (WT adjuvant and Nod2−/− IRBP) or 8 (WT IRBP and Nod2−/− adjuvant) mice; ***p < 0.0001. e Uveitis was scored by histopathology 21 days post-immunization. Data was combined from 6 independent experiments where n = 38 (WT) and 39 (Nod2−/−) mice; ***p < 0.0001. f Representative H&E-stained sections of the anterior segment (top) and posterior segment (bottom) of eyes day 21 post immunization. Pathological features indicated: retinal folding (arrowhead), granulomatous formation (asterisk), vasculitis (arrow), and severe photoreceptor damage (bracket). I, iris; CB, ciliary body; V, vitreous; R, retina. g Apoptosis within the retina (14 days post immunization) was determined by a TUNEL-based assay. Data was combined from 2 independent experiments where n = 8 (WT) and 7 (Nod2−/−) mice; ***p = 0.0003. h Leukocyte infiltration in the aqueous of the anterior segment (AS) and vitreous of the posterior segment (PS) were enumerated. Data are combined from 6 independent experiments where n = 35 (WT AS, WT PS), 39 (Nod2−/−AS), 42 (Nod2−/− PS) mice; **p = 0.0011 (AS) and ***p = 0.0005 (PS). Data are mean ± SEM (b, e, h) with dots representing individual values (e, h) or box-whisker plots with median, 25–75th percentile and min–max range (d, g). Source data are provided as a Source Data file.