Fig. 4. Immunization scheme and raw data of HK-B immunized mice.

a Frequency of IgM- and IgG-SCs in mice immunized with alum alone or different HK-B. b Ratio of class-switched IgG over IgM-SCs. c Frequency of bacteria-binding IgG-SCs in the different immunizations. d Characterization of HK-B binding IgG in HK-ST immunized (left, and HK-EB immunized mice (right). Data are cumulated from all three immunized mice (for each panel 200 > n_{anti-HK-B IgG-SCs} > 50), binned as described in the methods and shown as a relative frequency of IgG in bin over total affine IgG-SCs. All frequency data (a–c) in this figure is shown as the mean frequency ± standard deviation over all mice (n = 3).