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. Author manuscript; available in PMC: 2020 Oct 27.
Published in final edited form as: Mol Cancer Res. 2013 Apr 17;11(7):689–698. doi: 10.1158/1541-7786.MCR-12-0673

Figure 1.

Figure 1.

FIR represses endogenous c-Myc with wild-type TFIIH/p89/XPB/ERCC3. A, both total FIRs (authentic FIR and FIR splicing variants including FIRΔexon2) and P89 expression were higher in colon cancer tissues (T) than in corresponding noncancer tissues (N). P < 0.005 by the Wilcoxon test, and P < 0.01 by the t test. B, FIR knockdowns by siRNA (5 or 20 nmol/L) shows reduced c-Myc, SAP155, and P89 expression in HeLa cells. Each protein expression was indicated as to the ratio to β-actin. C, SAP155 siRNA (5, 10, or 25 nmol/L) suppressed P89 expression but increased c-Myc expression in HeLa cells, as well as shifting the FIR bands (arrows) and decreasing SAP130 expression. D, P89, total FIR, and SAP155 are more strongly expressed in excised human colorectal cancer tissues (arrows) than in normal colon mucosa. Hemotoxylin and eosin (H&E) staining of colorectal cancer tissues and normal colon mucosa is also shown.