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. 2020 Oct 26;10:18215. doi: 10.1038/s41598-020-75209-z

Figure 3.

Figure 3

Visual representation of the effect of chia seeds and SRD during acquisition and reversal learning phase by exemplary pseudocolor coded heatmaps showing presence probabilities of ADV mice in the MWM. Reddish tones denote high presence probabilities and bluish tones denote low presence probabilities. The left panel indicates the day, the platform position (black dot) and the starting position of mice (arrow). The first row of heatmaps shows the presence probabilities of WT mice receiving CD + CD. The second row of heatmaps represents the presence probabilities of ADV APP23 mice receiving CD + CD, showing notably less directed swimming trajectories. The third row of heatmaps represents the presence probabilities of ADV APP23 mice receiving CD + Chia. Day 2 and 3 (marked by gray box) show more targeted swimming trajectories, compared to APP23 mice on CD + CD. The last row of heatmaps represents the presence probabilities of ADV APP23 mice receiving SRD + SRD. Day 2 and 3, as well as day 4 and 5 (marked by gray boxes), show more targeted swimming trajectories compared to APP23 mice receiving CD + CD. Heatmaps were generated with Matlab R2011b as follows. First, for each mouse swim paths of all 6 trials per day were projected to planar space as binary images (swim trajectory was set to value 1, non-swim path was set to value 0). Second, a single swim path per mouse and day was obtained using the mathematical union operation with values set to 1, if the animal traversed this position in at least 1 out of 6 trials. Third, probabilistic maps were created based on the binary images of all mice in one group on one day, such that the probability of 1 was assigned to a pixel that was crossed by every mouse in the respective group on the respective day. Conversely, a probability of 0 was allocated to a pixel that was never traversed by any mouse in the respective group on the respective day. WT wild type control, APP23 transgenic mouse model, PRE pre-plaque stage, ADV advanced-plaque stage, CD control, SRD sucrose-rich, Chia chia seed supplementation.