Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 21;9(10):2333. doi: 10.3390/cells9102333

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Confocal images and flow cytometry analysis of the acidic vesicles formed in the cytoplasm of control HeLa cells (ShWT1) and tafazzin knockdown cells (ShTaz1). (a–c) Analysis of the three HeLa cell lines (ShWT1, ShTaz1 and ShTaz1R) stained with AO. (a) Acridine orange staining of the lysosomes and other acidic compartments. At the top, ShWT1 control cells and bottom, ShTaz1 tafazzin knockdown. (b) Histogram representation of the number of red vesicles per nucleus. Calculation from 10–20 image field as presented in Figure 6a,b. (c) Histogram representation of vesicle size in μM calculated in the same image fields as presented in Figure 6a,b. (d) Flow cytometry analysis of the accumulation of LysoTracker Green in arbitrary units (a.u.). (e) Flow cytometry analysis of staining by monodansylcadaverine (MDC, in arbitrary units). ns = no significant and *** is the p-value ≤ 0.001.