Table 2.
Mitochondrial activities measured in intact permeabilized HeLa cell lines (ShWT1, ShTaz1, and ShTaz1R).
| Activities. nmol/min/mg |
ATP Synthesis Activity | Citrate Synthase Activity | ATP/CS |
|---|---|---|---|
| ShWT1 | 25.8 ± 1.5 | 182.3 ± 24.6 | 0.141 |
| ShTaz1 | 17.4 ± 1.2 * | 287.5 ± 9.0 * | 0.060 * |
| ShTaz1R | 23.9 ± 1.4 | 201.6 ± 19.3 | 0.118 |
| Coupled Respiration | Uncoupled Respiration | CS/Coupled | |
| ShWT1 | 7.7 ± 0.6 | 19.7 ± 0.8 | 0.042 ± 0.004 |
| ShTaz1 | 6.9 ± 0.9 | 19.6 ± 1.8 | 0.024 ± 0.006 * |
| ShTaz1R | 7.5 ± 0.6 | 19.8 ± 1.3 | 0.037 ± 0.007 |
ATP synthesis activity and citrate synthase activity (CS). The values shown are means of at least three independent measurements expressed in nmol/min/mg protein. Statistically significant differences between ShWT1 control and ShTaz1 or HeLaTaz1R cells are indicated: * p < 0.05.